LOCUS       SPLICIFY           9906 bp    DNA     circular   04-FEB-2026
DEFINITION  Sequence exported from Splicify
SOURCE      synthetic
  ORGANISM  synthetic construct

FEATURES             Location/Qualifiers
     source          1..9906
                     /organism="synthetic construct"
                     /mol_type="genomic DNA"
     misc_feature    1..589
                     /label="ori"
     misc_feature    490..509
                     /label="pBR322ori-F"
     misc_feature    682..699
                     /label="L4440"
     misc_feature    complement(8031..8279)
                     /label="U6 promoter"
     misc_feature    complement(8089..8108)
                     /label="LKO.1 5'"
     misc_feature    complement(8259..8279)
                     /label="hU6-F"
     misc_feature    complement(8409..8428)
                     /label="T7"
     misc_feature    complement(8410..8428)
                     /label="T7 promoter"
     misc_feature    complement(8439..8456)
                     /label="M13 Forward"
     misc_feature    complement(8439..8455)
                     /label="M13 fwd"
     misc_feature    complement(8448..8470)
                     /label="M13/pUC Forward"
     misc_feature    8771..8875
                     /label="AmpR promoter"
     misc_feature    8876..9736
                     /label="AmpR"
     misc_feature    complement(9094..9113)
                     /label="Amp-R"
     misc_feature    7865..7865
                     /label="deletion (2029 bp removed)"
     misc_feature    700..7864
                     /label="added sequence"

ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcattagg caccccaggc
      661 tttacccgaa cgaccgagcg cagcgagtca gtgagcgaga aacagctatg accatgatta
      721 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctcg gatctgctgg
      781 gcttgctgaa ggtagggggt caagaaccag aggtgtaaag tacttgagta attttacttg
      841 attactgtac ttaagtatta tttttgggga tttttacttt acttgagtac aattaaaaat
      901 caatactttt acttttactt aattacattt ttttagaaaa aaaagtactt tttactcctt
      961 acaattttat ttacagtcaa aaagtactta ttttttggag atcacttgaa ttctgtggaa
     1021 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     1081 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     1141 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     1201 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     1261 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     1321 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     1381 cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa
     1441 tacgacaagg tgaggaacta aaccatggcc aagcctttgt ctcaagaaga atccaccctc
     1501 attgaaagag caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc
     1561 agcgcagctc tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact
     1621 gggggacctt gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac
     1681 ctgacttgta tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg
     1741 tgccgacagg tgcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat
     1801 ggacagccga cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc
     1861 taagcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc
     1921 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     1981 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     2041 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     2101 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtttcaggct
     2161 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agcccggatc
     2221 cttatcgcga ttttaccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacatc
     2281 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     2341 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     2401 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgctcga
     2461 agcggccgct ccccagcatg cctgctattc tcttcccaat cctccccctt gctgtcctgc
     2521 cccaccccac cccccagaat agaatgacac ctactcagac aatgcgatgc aatttcctca
     2581 ttttattagg aaaggacagt gggagtggca ccttccaggg tcaaggaagg cacgggggag
     2641 gggcaaacaa cagatggctg gcaactagaa ggcacagtcg aggctgatca gcgagctcta
     2701 ggaattctta ctttttcttt tttgcctggc cggccttttt cgtggccgcc ggccttttgt
     2761 cgcctcccag ctgagacagg tcgatccgtg tctcgtacag gccggtgatg ctctggtgga
     2821 tcagggtggc gtccagcacc tctttggtgc tggtgtacct cttccggtcg atggtggtgt
     2881 caaagtactt gaaggcggca ggggctccca gattggtcag ggtaaacagg tggatgatat
     2941 tctcggcctg ctctctgatg ggcttatccc ggtgcttgtt gtaggcggac agcactttgt
     3001 ccagattagc gtcggccagg atcactctct tggagaactc gctgatctgc tcgatgatct
     3061 cgtccaggta gtgcttgtgc tgttccacaa acagctgttt ctgctcatta tcctcggggg
     3121 agcccttcag cttctcatag tggctggcca ggtacaggaa gttcacatat ttggagggca
     3181 gggccagttc gtttcccttc tgcagttcgc cggcagaggc cagcattctc ttccggccgt
     3241 tttccagctc gaacagggag tacttaggca gcttgatgat caggtccttt ttcacttctt
     3301 tgtagccctt ggcttccaga aagtcgatgg gattcttctc gaagctgctt ctttccatga
     3361 tggtgatccc cagcagctct ttcacactct tcagtttctt ggacttgccc ttttccactt
     3421 tggccaccac cagcacagaa taggccacgg tggggctgtc gaagccgccg tacttcttag
     3481 ggtcccagtc cttctttctg gcgatcagct tatcgctgtt cctcttgggc aggatagact
     3541 ctttgctgaa gccgcctgtc tgcacctcgg tctttttcac gatattcact tggggcatgc
     3601 tcagcacttt ccgcacggtg gcaaaatccc ggcccttatc ccacacgatc tccccggttt
     3661 cgccgtttgt ctcgatcaga ggccgcttcc ggatctcgcc gttggccagg gtaatctcgg
     3721 tcttgaaaaa gttcatgatg ttgctgtaga agaagtactt ggcggtagcc ttgccgattt
     3781 cctgctcgct cttggcgatc atcttccgca cgtcgtacac cttgtagtcg ccgtacacga
     3841 actcgctttc cagcttaggg tactttttga tcagggcggt tcccacgacg gcgttcaggt
     3901 aggcgtcgtg ggcgtggtgg tagttgttga tctcgcgcac tttgtaaaac tggaaatcct
     3961 tccggaaatc ggacaccagc ttggacttca gggtgatcac tttcacttcc cggatcagct
     4021 tgtcattctc gtcgtactta gtgttcatcc gggagtccag gatctgtgcc acgtgctttg
     4081 tgatctgccg ggtttccacc agctgtctct tgatgaagcc ggccttatcc agttcgctca
     4141 ggccgcctct ctcggccttg gtcagattgt cgaactttct ctgggtaatc agcttggcgt
     4201 tcagcagctg ccgccagtag ttcttcatct tcttcacgac ctcttcggag ggcacgttgt
     4261 cgctcttgcc ccggttcttg tcgcttctgg tcagcacctt gttgtcgatg gagtcgtcct
     4321 tcagaaagct ctgaggcacg atatggtcca catcgtagtc ggacagccgg ttgatgtcca
     4381 gttcctggtc cacgtacata tcccgcccat tctgcaggta gtacaggtac agcttctcgt
     4441 tctgcagctg ggtgttttcc acggggtgtt ctttcaggat ctggctgccc agctctttga
     4501 tgccctcttc gatccgcttc attctctcgc ggctgttctt ctgtcccttc tgggtggtct
     4561 ggttctctct ggccatttcg atcacgatgt tctcgggctt gtgccggccc atcactttca
     4621 cgagctcgtc caccaccttc actgtctgca ggatgccctt cttaatggcg gggctgccgg
     4681 ccagattggc aatgtgctcg tgcaggctat cgccctggcc ggacacctgg gctttctgga
     4741 tgtcctcttt aaaggtcagg ctgtcgtcgt ggatcagctg catgaagttt ctgttggcga
     4801 agccgtcgga cttcaggaaa tccaggattg tcttgccgga ctgcttgtcc cggatgccgt
     4861 tgatcagctt ccggctcagc ctgccccagc cggtgtatct ccgccgcttc agctgcttca
     4921 tcactttgtc gtcgaacagg tgggcatagg ttttcagccg ttcctcgatc atctctctgt
     4981 cctcaaacag tgtcagggtc agcacgatat cttccagaat gtcctcgttt tcctcattgt
     5041 ccaggaagtc cttgtccttg ataattttca gcagatcgtg gtatgtgccc agggaggcgt
     5101 tgaaccgatc ttccacgccg gagatttcca cggagtcgaa gcactcgatt ttcttgaagt
     5161 agtcctcttt cagctgcttc acggtcactt tccggttggt cttgaacagc aggtccacga
     5221 tggccttttt ctgctcgccg ctcaggaagg cgggctttct cattccctcg gtcacgtatt
     5281 tcactttggt cagctcgtta tacacggtga agtactcgta cagcaggctg tgcttgggca
     5341 gcaccttctc gttgggcagg ttcttatcga agttggtcat ccgctcgatg aagctctggg
     5401 cggaagcgcc cttgtccacc acttcctcga agttccaggg ggtgatggtt tcctcgctct
     5461 ttctggtcat ccaggcgaat ctgctgtttc ccctggccag agggcccacg tagtagggga
     5521 tgcggaaggt caggatcttc tcgatctttt cccggttgtc cttcaggaat gggtaaaaat
     5581 cttcctgccg ccgcagaatg gcgtgcagct ctcccaggtg gatctggtgg gggatgctgc
     5641 cgttgtcgaa ggtccgctgc ttccgcagca ggtcctctct gttcagcttc acgagcagtt
     5701 cctcggtgcc gtccatcttt tccaggatgg gcttgatgaa cttgtagaac tcttcctggc
     5761 tggctccgcc gtcaatgtag ccggcgtagc cgttcttgct ctggtcgaag aaaatctctt
     5821 tgtacttctc aggcagctgc tgccgcacga gagctttcag cagggtcagg tcctggtggt
     5881 gctcgtcgta tctcttgatc atagaggcgc tcaggggggc cttggtgatc tcggtgttca
     5941 ctctcaggat gtcgctcagc aggatggcgt cggacaggtt cttggcggcc agaaacaggt
     6001 cggcgtactg gtcgccgatc tgggccagca ggttgtccag gtcgtcgtcg taggtgtcct
     6061 tgctcagctg cagtttggca tcctcggcca ggtcgaagtt gctcttgaag ttgggggtca
     6121 ggcccaggct cagggcaatc aggtttccga acaggccatt cttcttctcg ccgggcagct
     6181 gggcgatcag attttccagc cgtctgctct tgctcagtct ggcagacagg atggccttgg
     6241 cgtccacgcc gctggcgttg atggggtttt cctcgaacag ctggttgtag gtctgcacca
     6301 gctggatgaa cagcttgtcc acgtcgctgt tgtcggggtt caggtcgccc tcgatcagga
     6361 agtggccccg gaacttgatc atgtgggcca gggccagata gatcagccgc aggtcggcct
     6421 tgtcggtgct gtccaccagt ttctttctca ggtggtagat ggtggggtac ttctcgtggt
     6481 aggccacctc gtccacgatg ttgccgaaga tggggtgccg ctcgtgcttc ttatcctctt
     6541 ccaccaggaa ggactcttcc agtctgtgga agaagctgtc gtccaccttg gccatctcgt
     6601 tgctgaagat ctcttgcaga tagcagatcc ggttcttccg tctggtgtat cttcttctgg
     6661 cggttctctt cagccgggtg gcctcggctg tttcgccgct gtcgaacagc agggctccga
     6721 tcaggttctt cttgatgctg tgccggtcgg tgttgcccag caccttgaat ttcttgctgg
     6781 gcaccttgta ctcgtcggtg atcacggccc agcccacaga gttggtgccg atgtccaggc
     6841 cgatgctgta cttcttgtcg gctgctggga ctccgtggat accgaccttc cgcttcttct
     6901 ttggggccat cttatcgtca tcgtctttgt aatcaatatc atgatccttg tagtctccgt
     6961 cgtggtcctt atagtccatg gtggcaccgg tccaacctga aaaaaagtga tttcaggcag
     7021 gtgctccagg taattaaaca ttaatacccc accaaccaac catcccttaa acccttacct
     7081 cttgctcagc taattacagc ccggaggaga agggccgtcc cgcccgctca cctgtgggag
     7141 taacgcggtc agtcagagcc ggggcgggcg gcgcgaggcg gcggcggagc ggggcacggg
     7201 gcgaaggcag cgtcgcagcg actcccgccc gccgcgcgct tcgcttttta tagggccgcc
     7261 gccgccgccg cctcgccata aaaggaaact ttcggagcgc gccgctctga ttggctgccg
     7321 ccgcacctct ccgcctcgcc ccgccccgcc cctcgccccg ccccgccccg cctggcgcgc
     7381 gccccccccc cccccccgcc cccatcgctg cacaaaataa ttaaaaaata aataaataca
     7441 aaattggggg tggggagggg ggggagatgg ggagagtgaa gcagaacgtg gggctcacct
     7501 cgaccatggt aatagcgatg agtaatacgt agatgtactg ccaagtagga aagtcccata
     7561 aggtcatgta ctgggcacaa tgccaggcgg gccatttacc gtcattgacg tcaatagggg
     7621 gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac cgtaaatact
     7681 ccacccattg acgtcaatgg aaagtcccta ttggcgttac tattgacgtc aatgggcggg
     7741 ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg ggtacccaat
     7801 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     7861 tggggaactc catatatggg ctatgaacta atgaccccgt aattgattac tattactaga
     7921 aaaaaagcac cgactcggtg ccactttttc aagttgataa cggactagcc ttatttaaac
     7981 ttgctatgct gtttccagca tagctcttaa acaggtcttc tcgaagaccc ggtgtttcgt
     8041 cctttccaca agatatataa agccaagaaa tcgaaatact ttcaagttac ggtaagcata
     8101 tgatagtcca ttttaaaaca taattttaaa actgcaaact acccaagaaa ttattacttt
     8161 ctacgtcacg tattttgtac taatatcttt gtgtttacag tcaaattaat tccaattatc
     8221 tctctaacag ccttgtatcg tatatgcaaa tatgaaggaa tcatgggaaa taggccctct
     8281 tcctgcccga ccttggtacc ggatccagtc gactgaattg gttcctttaa agcctgcttt
     8341 tttgtacaga attctcgagt ggcgcgcctg gacaagacac gtggccttga cggccttccg
     8401 ccaattcgcc ctatagtgag tcgtattacg tcgcgctcac tggccgtcgt tttacaacgt
     8461 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     8521 gccagctggc gtaatagcga agaggcccgc accgaaacgc ccttcccaac agttgcgcag
     8581 cctgaatggc gaatgggagc gccctgtagc ggccactcaa ccctatctcg gtctattctt
     8641 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     8701 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
     8761 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     8821 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     8881 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     8941 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     9001 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     9061 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     9121 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     9181 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     9241 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     9301 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     9361 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     9421 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     9481 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     9541 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg gttctcgcgg
     9601 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     9661 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     9721 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     9781 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     9841 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     9901 atcttc
//