Splicify · Clone v1.0
Splicify - Clone logo

Splicify - Clone

Plasmid cloning reimagined

Design a Gibson assembly to build my target plasmid from inventory
Plasmid Visualizer
Length: 523 bpAnnotations: 12
Tip: Select sequence and press Delete to remove. Use "Add / Change Sequence" to insert or modify sequence.
5′ … 1
1
10203040GCCTCCTGCTGGTCCCAAGTTGTGAAATCTTTATCGTGTTTGoverlap FragmeFragment_3 REVFragment_1 FWD primer (38bp)Fragment_1CGGAGGACGACCAGGGTTCAACACTTTAGAAATAGCACAAAC
42
43
50607080GTCAGTTCCAGGCGATGTTCAACGAAATGAAATTCTGGCAGCFragment_1CAGTCAAGGTCCGCTACAAGTTGCTTTACTTTAAGACCGTCG
84
85
90100110120TTGACAGGTTTTTTGGCTTTATACGTCGTTTTGAAATCAACAFragment_1AACTGTCCAAAAAACCGAAATATGCAGCAAAACTTTAGTTGT
126
127
130140150160CGATGATGACCGCCTCCTTTCACTTGCCTTCACCTTTACCCTFragment_1Fragment_1 REV primer (44bp)overlap Fragment_1-Fragment_2 (30bp, designed)Fragment_2 FWD primer (34bp)Fragment_2GCTACTACTGGCGGAGGAAAGTGAACGGAAGTGGAAATGGGA
168
168 … 3′
Annotations (12)
Note: Double-clicking split annotations (spanning lines or origin) may select the wrong annotation. Use this panel for reliable editing.